JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 3 (2016): Journal of the Chilean Chemical Society
Original Research Papers

SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA

Sarfaraz Ahmad
Department of pharmacy, Jazan university
Masoom R. Siddiqui
Advanced Materials Research Chair, Department of chemistry, College of Science, King Saud University
MD. S. Ali
Department of pharmacy, Jazan university
Saikh M. Wabaidur
Advanced Materials Research Chair, Department of chemistry, College of Science, King Saud University
MD. S. Alam
Department of pharmacy, Jazan university
Nawazish Alam
Department of pharmacy, Jazan university
Zeid A. Alothman
Advanced Materials Research Chair, Department of chemistry, College of Science, King Saud University
Moonis Ali Khan
Advanced Materials Research Chair, Department of chemistry, College of Science, King Saud University
Mohammad R. Khan
Advanced Materials Research Chair, Department of chemistry, College of Science, King Saud University
Published September 10, 2016
Keywords
  • Venlafaxine,
  • O-desmethyl venlafaxine,
  • Pharmacokinetics,
  • Solid phase extraction,
  • LC-MS/MS
How to Cite
Ahmad, S., Siddiqui, M. R., Ali, M. S., Wabaidur, S. M., Alam, M. S., Alam, N., Alothman, Z. A., Khan, M. A., & Khan, M. R. (2016). SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA. Journal of the Chilean Chemical Society, 61(3). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/93

Abstract

A rapid, simple, sensitive LC-MS/MS method involving a least pretreatment process has been proposed for the quantitative assessment of venlafaxine (VEN) and O-desmethyl venlafaxine (ODV) using cetirizine as an internal standard. The method was validated over the range of 1.03 ng/mL to 453.50 ng/mL (venlafaxine) and 1.32 ng/mL to 585.21 ng/mL (O-desmethyl venlafaxine). The lowest limit of quantification for venlafaxine and O-desmethyl venlafaxine was found to be 1.03 ng/mL and 1.32 ng/mL, respectively. The solid phase extraction procedure provided reliable and reproducible recoveries of the drug as well as its active metabolite with no interference at their retention time. The recovery for all analyzed drugs was found to be in the range of 72.55% to 74.75%. The result indicates that the developed procedure could be considered suitable for carry out simultaneous preclinical pharmacokinetics studies for VEN and ODV. 

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