JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 63 No 2 (2018): Journal of the Chilean Chemical Society
Original Research Papers

SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH

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  • Paulo A. B. Da Silva,
  • Gustavo C. S. De Souza,
  • Ana Paula S. Paim,
  • André F. Lavorante
Paulo A. B. Da Silva
Instituto Federal de Alagoas, Campus Coruripe Departamento de Química Fundamental, Universidade Federal de Pernambuco
Gustavo C. S. De Souza
Departamento de Química Fundamental, Universidade Federal de Pernambuco
Ana Paula S. Paim
Departamento de Química Fundamental, Universidade Federal de Pernambuco
André F. Lavorante
Departamento de Química, Universidade Federal Rural de Pernambuco
Published June 25, 2018
Keywords
  • Dialysis,
  • Flow injection analysis,
  • Multicommutation,
  • Reducing sugar,
  • Wine
How to Cite
Da Silva, P. A. B., De Souza, G. C. S., Paim, A. P. S., & Lavorante, A. F. (2018). SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH. Journal of the Chilean Chemical Society, 63(2). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/684

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Abstract

A multicommuted flow procedure for the spectrophotometric determination of reducing sugars in wine has been developed. A dialysis unit was incorporated in the system to minimize matrix interferences and eliminate the colored compounds of the wine. The oxidation-reduction reaction in an alkaline medium between reducing sugars and the Cu2+-Neocuproine complex was employed. The compound formed was monitored at 460 nm. In the proposed procedure, there was a linear response between 0.25 and 4.0 g L-1 reducing sugars (y = (0.36 ± 0.02) + (0.31 ± 0.01) * x, R = 0.9975, n = 6), a coefficient of variance of 2.6% for a reducing sugar solution of 2.0 g L-1 (n = 7), a limit of detection estimated at 0.03 g L-1, reagent consumption per determination of 0.330, 0.128 and 0.160 mg for neocuproine, CuSO4 and NaOH, respectively. A sampling throughput of 45 determinations per hour was achieved. The proposed system was applied to wine samples and comparing the results with an AOAC official method no significant difference at a 95% confidence level.

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