DETERMINATIONOF CATECHIN AS BIOMARKER IN ENTOCID POLYHERBAL DIGESTIVE SYRUP BY HPLC/DAD

Abstract

Entocid syrup is a balanced composition of vigilantly selected and formulated with effective herbs of proven efficacy in intestinal ailments covering the segment of hyper-acidity symptoms. Entocid is scientifically formulated in such a way that it not only neutralizes the acid secretion but at the same time it strengthens the stomach thus acts promptly and relieves the symptoms of GI upsets and in the long-run prevents the recurrence of such symptoms. The ingredients of Entocid syrup are rich in alkaloids, proteins, vitamins, glycosides, bioflavonoid and trace elements, formulated using ten potent herbal drugs namely Amomum sabulatum, Berberis aristata, Cinnamomum tamala, Coriandrum sativum, Cuminum cyminum, Foeniculum vulgare, Vitis vinifera, Mesua ferrea, Glycyrrhiza glabra and Mentha piperita. The flavonoid compounds exhibit preventive effects from colon carcinogenesis, lipid lowering effects, anti-oxidant, anti-inflammatory, anti-depressant and anti-atherogenic properties. To develop the assay method, high-performance liquid chromatography (HPLC) with a diode-array detector (DAD) was used. Using HPLC/DAD, catechin was eluted with gradient program. The UV characteristic of catechin, the detection wavelength was monitored at 265 nm. Method validation including limit of quantitation, the accuracy, inter and intra day and limitation was performed by using recovery tests. The future development of flavonoids-based drugs is limitation and assumes to provide significant effects on digestion related diseases.