JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 2 (2016): Journal of the Chilean Chemical Society
Original Research Papers

CHARACTERIZATION OF TEMPORAL BIODEGRADATION OF RADIATA PINE BY GLOEOPHYLLUM TRABEUM THROUGH PRINCIPAL COMPONENT ANALYSIS-BASED TWO-DIMENSIONAL CORRELATION FTIR SPECTROSCOPY

Claudio Pozo
Centro de Investigación de Polímeros Avanzados (CIPA) CONICYT- REGIONAL R08C1002. Beltrán Mathieu 224 piso 2, Concepción
Judith Díaz-Visurraga
Centro de Investigación de Polímeros Avanzados (CIPA) CONICYT- REGIONAL R08C1002. Beltrán Mathieu 224 piso 2, Concepción
David Contreras
Centro de Biotecnología, Universidad de Concepción Facultad de Ciencias Químicas, Universidad de Concepción. Edmundo Larenas 129, Casilla 160-C, Concepción
Juanita Freer
Centro de Biotecnología, Universidad de Concepción Facultad de Ciencias Químicas, Universidad de Concepción. Edmundo Larenas 129, Casilla 160-C, Concepción
Jaime Rodríguez
Centro de Biotecnología, Universidad de Concepción. Facultad de Ciencias Forestales, Universidad de Concepción. Victoria 631, Casilla 160-C, Concepción
Vol 61, No 2 (2016): Journal of the Chilean Chemical Society
Published June 10, 2016
Keywords
  • FTIR-PCA2D correlation spectroscopy,
  • Brown rot,
  • Gloeophyllum trabeum
How to Cite
Pozo, C., Díaz-Visurraga, J., Contreras, D., Freer, J., & Rodríguez, J. (2016). CHARACTERIZATION OF TEMPORAL BIODEGRADATION OF RADIATA PINE BY GLOEOPHYLLUM TRABEUM THROUGH PRINCIPAL COMPONENT ANALYSIS-BASED TWO-DIMENSIONAL CORRELATION FTIR SPECTROSCOPY. Journal of the Chilean Chemical Society, 61(2). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/6

Abstract

Brown rot fungi produce a special pattern of wood decay. In the first stages of biodegradation, fast depolymerisation of holocellulose causes a rapid loss of wood strength while lignin is not substantially depolymerized. Fourier Transform Infrared Spectroscopy (FTIR) is a powerful tool for examining changes in wood chemistry. FTIR is an accurate, non-destructive technique, which usually requires a minimum of sample preparation and sample size. Principal component analysis-based two-dimensional (PCA2D) correlation spectroscopy of a spectral data set was applied to assess temporal brown-rot wood decay. Pinus radiata with soil support was degraded by brown rot fungi Gloeophyllum trabeum for a period of 0, 1, 2, 3, 4, 8, 12 and 16 weeks simulating the natural process of biodegradation. Decayed samples were monitored and analyzed periodically by FTIR. Most of the autopeaks had contributions from lignin, cellulose, and hemicelluloses. The main effect observed by PCA2D correlation was the significant decrease in the intensities of bands predominantly in the region of 2000-1000 cm-1, associated with polysaccharides in the biodegraded wood. An increase in the intensities of bands at 1698 and 1664 cm-1associated with the oxidation of wood components, mostly lignin, was also observed. Detailed analysis of asynchronous map showed that the wood oxidation began after the depolymerisation of polysaccharides.

Figure 1. Reconstructed data of the FTIR spectra of the wood sample studied from loading vectors and scores of PC1, PC2 and PC3 in 1800–800 cm-1. Arrows indicate the directions of the changes in the intensity bands with increasing of biodegradation time.

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