JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 1 (2016): Journal of the Chilean Chemical Society
Original Research Papers

PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)

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  • Abdul Qayyum,
  • Raja Adil Sarfraz,
  • Aisha Ashraf,
  • Shazia Adil
Abdul Qayyum
Department of Chemistry University of Agriculture, Faisalabad, (38040)
Raja Adil Sarfraz
Department of Chemistry University of Agriculture, Faisalabad, (38040) Central Hi-Tech Laboratory, University of Agriculture, Faisalabad, (38040)
Aisha Ashraf
Department of Chemistry, Gout. College Women University, Faisalabad, (38040)
Shazia Adil
Department of Chemistry University of Agriculture, Faisalabad, (38040)
Published December 10, 2015
Keywords
  • Antidiabetic,
  • DPPH,
  • Heliotropium Strigosum,
  • Solvent fractions
How to Cite
Qayyum, A., Adil Sarfraz, R., Ashraf, A., & Adil, S. (2015). PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM). Journal of the Chilean Chemical Society, 61(1). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/52

Copyright (c) 2016 Abdul Qayyum, Raja Adil Sarfraz, Aisha Ashraf, Shazia Adil

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Abstract

Heliotropium strigosum plant is widely used in traditional medicines for treatment of various ailments. In current study, an effort was made to evaluate phenolic profile as well as antidiabetic and antioxidant activities of crude methanol extract and different solvent (n-hexane, ethyl acetate, chloroform and aqueous) fractions of H. strigosum. Total phenolic contents were determined by spectrophotometric assay. Polyphenolic compounds in crude methanol extract and each solvent fraction were identified by reverse phase high performance liquid chromatography (RP-HPLC). Antioxidant and antidiabetic activities were determined using DPPH and α-amylase inhibition assays, respectively. The outcome of spectrophotometric assay showed that methanol extract had higher amount of total phenolic (84.50 ± 0.06 μg GAE/ mg of plant extract) contents than aqueous, ethyl acetate, chloroform and n-hexane fractions, respectively. The RP-HPLC analysis revealed the maximum number of phenolic (chromotropic acid, quercetin, trans-4-hydroxy-3-methoxy cinnamic acid, vanillic acid, gallic acid, caffeic acid, m-coumaric acid, p-coumaric acid, syringic acid, sinapic acid and ferulic acid) compounds in methanol extract. Methanol extract (IC50 = 8.97 μg/mL) exhibited the maximum antidiabetic activity followed by aqueous (IC50 = 20.04 μg/mL), ethyl acetate (IC50 = 27.79 μg/mL), chloroform (IC50 = 56.87 μg/mL) and hexane (IC50 = 32.16 μg/mL) fractions, respectively. The outcome of antioxidant assay revealed that methanol extract was the leading one with regard to antioxidant activity at different doses (10 to 250 μg/mL). The current study concludes that H. strigosum solvents extracts with significant phenolic profile and potent biological activities could be explored for potential uses in neutraceutical and pharmaceutical industries.

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