JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 2 (2016): Journal of the Chilean Chemical Society
Original Research Papers

DEVELOPMENT AND VALIDATION OF SIMPLE, RAPID AND SENSITIVE UV, HPLC AND HPTLC METHODS FOR THE ESTIMATION OF PIRFENIDONE IN TABLET DOSAGE FORM

S. G. Thorat
Department of Pharmaceutical Chemistry, Gurunanak College of Pharmacy, Dixit Nagar, Near CP Foundry, Nari, Nagpur - 440 026 (MS) Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440 033 (MS)
S. P. Padmane
Department of Pharmaceutical Chemistry, Gurunanak College of Pharmacy, Dixit Nagar, Near CP Foundry, Nari, Nagpur - 440 026 (MS)
M. R. Tajne
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440 033 (MS)
A. M. Ittadwar
Department of Pharmaceutical Chemistry, Gurunanak College of Pharmacy, Dixit Nagar, Near CP Foundry, Nari, Nagpur - 440 026 (MS)
Vol 61, No 2 (2016): Journal of the Chilean Chemical Society
Published June 10, 2016
Keywords
  • UV,
  • HPLC,
  • HPTLC,
  • Pirfenidone,
  • Anti-fibrotic agent
How to Cite
Thorat, S. G., Padmane, S. P., Tajne, M. R., & Ittadwar, A. M. (2016). DEVELOPMENT AND VALIDATION OF SIMPLE, RAPID AND SENSITIVE UV, HPLC AND HPTLC METHODS FOR THE ESTIMATION OF PIRFENIDONE IN TABLET DOSAGE FORM. Journal of the Chilean Chemical Society, 61(2). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/26

Abstract

UV, HPLC and HPTLC methods were developed and validated for the quantitative determination of Pirfenidone, a novel antifibrotic agent used in idiopathic pulmonary fibrosis. Chromatography was carried out by isocratic technique on reversed phase Eclipse XDB-C18 column (150 x 4.6 mm, 5μm) with mobile phase consisting of phosphate buff: acetonitrile (pH 3.5) 72:28 v/v at flow rate 1mL/min. TLC was carried out by stationary phase precoated Silica Gel 60 F254 TLC Plate using mobile phase Toluene: Methanol, 8:2 v/v. The UV spectrophotometric determination was performed at 311nm using solvent methanol. The proposed methods were validated according to ICH Q2-(R1) guidelines. The linearity range for Pirfenidone was 5-70 μg/mL for HPLC, 800-1600 ng/spot for HPTLC and 10-60 μg/mL for UV method. These methods were accurate and precise with recoveries in the range of 98.2-102.32 and relative standard deviation < 2%. The developed methods were successfully applied for determination of Pirfenidone in tablets.

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