Vol 67 No 2 (2022): Journal of the Chilean Chemical Society
Original Research Papers


Sigrid Mennickent
Universidad de Concepción
Esteban Pino
Universidad de Concepción
Published June 24, 2022
  • Carbamazepine,
  • Saliva,
  • Validation
How to Cite
de Diego, M., Mennickent, S., Pino, E., & Correa, D. (2022). A VALIDATED LC METHOD FOR DETERMINATION OF CARBAMAZEPINE IN SALIVA. Journal of the Chilean Chemical Society, 67(2), 5496-5499. Retrieved from


Carbamazepine (CBZ) is a drug used as anticonvulsant, especially in the treatment of epilepsy. For adequate control disease, treatment must achieve adequate blood concentrations, therefore, it is very important to control the levels of anticonvulsant drugs in biological matrices with suitable analytical methods. A simple and fast liquid chromatographic method with UV detection was developed and validated for the determination of CBZ in saliva. Chromatographic separation was achieved with a RP-18 column, using acetonitrile and water with triethylamine at pH 7,3 as mobile phase in isocratic elution mode, at a flow rate of 1 mL/min. The detection was done at 230 nm. and bromazepam at 1,5 µg/mL was used as IS. The run time was 5 min. The described method was linear over a range of 0,5 - 5,5 mg/mL. The intraassay and interassay precision, expressed as the RSD, were in the range of 0,67 - 2,93 % and 0,17 - 9,33 %, respectively, the extraction recoveries were between 100,67 – 100,69 %, the accuracy values ranged from -3,33 to 0,69 % and - 2,67 to 0,72 in intraday and interday analysis, respectively, and the assay demonstrated adequate selectivity and specificity. The LLOQ is below the therapeutic level, demonstrating an adequate sensitivity of the method. The results showed that the proposed method was found to be suitable for quantitative determination of CBZ in saliva.



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