VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF NORTRIPTYLINE AND PREGABALIN IN BULK AND COMBINED DOSAGE FORMULATIONS
- Nortriptyline,
- Pregabalin,
- HPLC,
- Stability indicating,
- Validation
Copyright (c) 2017 Haritha Potluri, Sreenivasa Rao Battula, Sunandamma Yeturu
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Abstract
A simple and precise stability indicating RP-HPLC method was developed for simultaneous analysis of drug nortriptyline and pregabalin using BDS (250mm x 4.6 mm, 5m) C18 column at 210 nm of UV detection. Perchloric acid (0.1%) and acetonitrile in the ratio of 55:45 was used as the mobile phase with a flow rate of 1.0 ml/min and linearity response was established over the concentration range of 5-30 μg/ml for nortriptyline and 37.5-225 μg/ml for pregabalin. The active pharmaceutical ingredients recovered for nortriptyline and pregabalin are in the range of 100.60-101.65% and 100.59-101.74% respectively. The method was validated and was found to be stability indicating and can be successfully utilized for the quantitative analysis of pharmaceutical tablet dosage formulations containing nortriptyline and pregabalin.
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