JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 62 No 2 (2017): Journal of the Chilean Chemical Society
Original Research Papers

CHEMICAL AND MICROSTRUCTURAL CHANGES IN EUCALYPTUS GLOBULUS FIBERS SUBJECTED TO FOUR DIFFERENT PRETREATMENTS AND THEIR INFLUENCE ON THE ENZYMATIC HYDROLYSIS

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  • Eduardo Troncoso,
  • Rosario Castillo,
  • Roberto Valenzuela,
  • Pablo Reyes,
  • Juanita Freer,
  • Marcela Norambuena,
  • Jaime Rodríguez,
  • Carolina Parra
Eduardo Troncoso
Biotechnology Center, University Campus, University of Concepción
Rosario Castillo
Biotechnology Center, University Campus, University of Concepción Faculty of Pharmacy, University Campus, University of Concepción
Roberto Valenzuela
Biotechnology Center, University Campus, University of Concepción
Pablo Reyes
Biotechnology Center, University Campus, University of Concepción
Juanita Freer
Biotechnology Center, University Campus, University of Concepción Faculty of Chemical Sciences, University Campus, University of Concepción
Marcela Norambuena
Biotechnology Center, University Campus, University of Concepción
Jaime Rodríguez
Biotechnology Center, University Campus, University of Concepción Faculty of Forest Sciences, University Campus, University of Concepción
Carolina Parra
Biotechnology Center, University Campus, University of Concepción
Published June 16, 2017
Keywords
  • Enzymatic hydrolysis,
  • Lignin micro-droplets,
  • Organosolv,
  • Kraft process,
  • Steam explosión,
  • Autohydrolysis,
  • Eucalyptus globulus
    • ...More
    Less
How to Cite
Troncoso, E., Castillo, R., Valenzuela, R., Reyes, P., Freer, J., Norambuena, M., Rodríguez, J., & Parra, C. (2017). CHEMICAL AND MICROSTRUCTURAL CHANGES IN EUCALYPTUS GLOBULUS FIBERS SUBJECTED TO FOUR DIFFERENT PRETREATMENTS AND THEIR INFLUENCE ON THE ENZYMATIC HYDROLYSIS. Journal of the Chilean Chemical Society, 62(2). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/183

Copyright (c) 2017 Eduardo Troncoso, Rosario Castillo, Roberto Valenzuela, Pablo Reyes, Juanita Freer, Marcela Norambuena, Jaime Rodríguez, Carolina Parra

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Abstract

In order to understand the relation between chemical composition, microscopic structure and enzymatic digestibility, different Eucalyptus globulus Wood pretreated samples were examined. Pretreated materials obtained by steam explosion and autohydrolysis were compared with those obtained by organosolv and kraft processes. Chemical analyses of pretreated materials showed a decrease in the content of xylans, except in the kraft pulp. FT-IR spectra showed that the residual lignin in autohydrolysis pulp had experienced greater changes compared to those in steam explosion and organosolv pulps, whereas minor changes in lignin kraft pulp were observed. The fiber morphology indicated that autohydrolysis pretreatment was the most aggressive treatment. Reduction in the content of lignin and its redistribution on the fiber wall were confirmed through confocal laser microscopy. The formation of discrete lignin droplets deposited on the surface of the fibers was observed in all pretreatments, with a higher frequency in organosolv followed by steam explosion. A significant increase in enzymatic accessibility was achieved in organosolv, autohydrolysis and steam explosion pulps, due to xylans removal combined with lignin redistribution. Homogeneous lignin distribution and higher xylan content may be related to the low enzymatic hydrolysis efficiency in kraft pulp.

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