JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 65 No 3 (2020): Journal of the Chilean Chemical Society
Original Research Papers

DETERMINATION OF ANTIOXIDANT CAPACITY (ORAC) OF GREIGIA SPHACELATA AND CORRELATION WITH VOLTAMMETRIC METHODS

Carlos G Peña-Farfal
Instituto de Ciencias Químicas Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Av. Alemania 01090, 4810101 - Temuco, CHILE
Published September 13, 2020
Keywords
  • Antioxidants,
  • Greigia sphacelata,
  • ORAC,
  • DPV

Abstract

The study and determination of the antioxidant capacity of the Greigia Sphacelata fruit were carried out. G. sphacelata is an endemic fruit of central-southern Chile, better known as Chupón or Quiscal. Spectrophotometric and modified ORAC test were developed for antioxidant capacity determinations, and later an electrochemical method was developed by differential impulse voltammetry (DPV) with a vitreous carbon electrode for the quantification of the antioxidant capacity expressed in equivalent of the Trolox standard in μmolL-1. Once the voltammetric test was developed, the correlation study between both methods used to determine the antioxidant capacity of Greigia sphacelata was carried out. The results of the determination of the antioxidant capacity of Greigia sphacelata by ORAC test report an antioxidant capacity of 3525 μmol/100 g equivalents of Trolox. Concerning the concentration obtained by the electrochemical methodology, 682 μmol/100 g Trolox equivalents is obtained for a linear correlation r = 0.969 with the spectrophotometric method.

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