JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 64 No 2 (2019): Journal of the Chilean Chemical Society
Original Research Papers

CONFOCAL MICROSCOPY STUDIES OF LIVING FUNGAL HYPHAE AND CONIDIA USING RHENIUM (I) TRICARBONYL COMPLEXES AS FLUORESCENT DYES

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  • Alexander Carreño,
  • Kamila Fernández,
  • Felipe Sáez-Cortez,
  • Carolina Otero,
  • Ramiro Arratia-Pérez,
  • Juan A. Fuentes,
  • Rubén Polanco
Alexander Carreño
Center for Applied Nanosciences (CANS) , Universidad Andres Bello
Kamila Fernández
Centro de Biotecnología Vegetal (CBV) , Facultad de Ciencias de la Vida, Universidad Andres Bello
Felipe Sáez-Cortez
Centro de Biotecnología Vegetal (CBV) , Facultad de Ciencias de la Vida, Universidad Andres Bello
Carolina Otero
Escuela de Química y Farmacia. Facultad de Medicina. Universidad Andres Bello
Ramiro Arratia-Pérez
Center for Applied Nanosciences (CANS) , Universidad Andres Bello
Juan A. Fuentes
Laboratorio de Genética y Patogénesis Bacteriana , Facultad de Ciencias de la Vida, Universidad Andres Bello
Rubén Polanco
Centro de Biotecnología Vegetal (CBV) , Facultad de Ciencias de la Vida, Universidad Andres Bello
Published July 25, 2019
How to Cite
Carreño, A., Fernández, K., Sáez-Cortez, F., Otero, C., Arratia-Pérez, R., Fuentes, J. A., & Polanco, R. (2019). CONFOCAL MICROSCOPY STUDIES OF LIVING FUNGAL HYPHAE AND CONIDIA USING RHENIUM (I) TRICARBONYL COMPLEXES AS FLUORESCENT DYES. Journal of the Chilean Chemical Society, 64(2). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/1208

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Abstract

Botrytis cinerea is a ubiquitous necrotrophic filamentous fungal pathogen, causing the “grey mold” disease in a wide range of plants, including species of importance in the food industry. Recently, we reported the use of fac-[Re(I)(CO)3(2,2’-bpy)L]PF6 (C3) and fac-[Re(I)(CO)3(4,4’-dimethyl-2,2’-bpy)L]PF6 (C4) complexes as biomarkers for yeasts and bacteria. Nevertheless, the use of these complexes to stain filamentous fungi, such as Botrytis cinerea, has not been explored so far. Since Botrytis cinerea exhibits a dynamic multilayer cell wall that changes the composition during normal growth, in both their hyphae and conidia, is not possible to directly extrapolate the use of both C3 and C4 as biomarkers for Botrytis cinerea without an experimental approach. In this work, we explored new features of the luminescent compounds C3 and C4 as biomarkers for the higher filamentous fungi Botrytis cinerea, including conidia and juvenile hyphae. We found that, with the new protocol proposed, both C3 and C4 were suitable to stain conidia with a simple procedure since no cell permeabilization is required. Additionally, these results suggest that C3 and C4 can selectively stain living conidia, allowing the differentiation from non-germinating conidia. This point opens a new focus of development for rhenium (I) tricarbonyl complexes as new fluorescent biomarkers for Botrytis cinerea with potential properties as vital staining.

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