JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 4 (2016): Journal of the Chilean Chemical Society
Original Research Papers

IMPLEMENTATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF INORGANIC ARSENIC SPECIES IN OCCUPATIONALLY EXPOSED HUMAN URINE SAMPLES AND ITS TOXIC EFFECTS ON EPITHELIAL CELLS OF RENAL COLLECTING TUBULE

Nicole Roldán
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso Laboratorio de Química Biológica, Pontificia Universidad Católica de Valparaíso
Nicolás Salinas Parra
Laboratorio de Química Biológica, Pontificia Universidad Católica de Valparaíso
Flavia Cifuentes Araneda
Laboratorio de Química Biológica, Pontificia Universidad Católica de Valparaíso
Helen Arias
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Manuel Bravo
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Waldo Quiroz
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Published December 10, 2016
Keywords
  • Arsenic,
  • Speciation,
  • Renal cells,
  • Urine,
  • Toxic effects

Abstract

In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L-1 and As(III) up to 10.7±0.6 μg L-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition. 

References

  1. K. S. Mohammed Abdul, S. S. Jayasinghe, E. P. S. Chandana, C. Jayasumana and P. M. C. S. De Silva, Environ. Toxicol. Pharmacol. 40, 828, (2015).
  2. B. K. Mandal and K. T. Suzuki, Talanta, 58, 201, (2002).
  3. W. R. Cullen and K. J. Reimer, Chemical Reviews 89, 713, (1989).
  4. L. Vega, M. Styblo, R. Patterson, W. Cullen, C. Wang and D. Germolec, Toxicol. Appl. Pharmacol. 172, 225 (2001).
  5. J. O. Nriagu, Arsenic in the Environment, Human Health and Ecosystem Effects, Wiley, 1994.
  6. S. A. D. Water, N. R. Council, C. L. Sciences and D. E. L. Studies, Arsenic in Drinking Water, National Academies Press, 1999.
  7. X. C. Le, X. Lu and X. F. Li, Anal. Chem., 76, 26A, (2004).
  8. L. Polissar, K. Lowry-Coble, D. A. Kalman, J. P. Hughes, G. van Belle, D. S. Covert, T. M. Burbacher, D. Bolgiano and N. K. Mottet, Environ. Res., 53, 29 (1990).
  9. K. Jomova, Z. Jenisova, M. Feszterova, S. Baros, J. Liska, D. Hudecova, C. J. Rhodes and M. Valko, J. Appl. Toxicol. 31, 95 (2011).
  10. A. Roy, M. M. Al-Bataineh and N. M. Pastor-Soler, Clin. J. Am. Soc. Nephrol. 10, 305 (2015).
  11. M. L. Robles-Osorio, E. Sabath-Silva and E. Sabath, Renal Fail. 37, 542 (2015).
  12. N. G. Docherty, A. I. Morales, J. M. López Novoa and F. Pérez Barriocanal, Nefrologia 27, 681 (2007).
  13. W. Quiroz, H. Arias, M. Bravo, M. Pinto, M. G. Lobos and M. Cortés, Microchem. J. 97, 78 (2011).
  14. Y. Bohari, A. Astruc, M. Astruc and J. Cloud, J. Anal. Atom. Spectrom. 16, 774 (2001).
  15. Y. Bohari, G. Lobos, H. Pinochet, F. Pannier, A. Astruc and M. Potin- Gautier, J. Environ. Monit. 4, 596 (2002).
  16. S. Parra, M. A. Bravo, W. Quiroz, T. Moreno, A. Karanasiou, O. Font, V. Vidal and F. Cereceda, Chemosphere 111, 513 (2014).
  17. W. Quiroz, H. Arias, M. Bravo, M. Pinto, M. G. Lobos and M. Cortés, Microchem. J. 97, 78 (2011).
  18. ACGIH, Documentation of the Biological Exposure Indices, 7th edn., 2001.
  19. Y. H. Hwang, Z. Y. Lee, J. D. Wang, Y. M. Hsueh, I. C. Lu and W. L. Yao, Environ. Res. 90, 207 (2002).
  20. J. Feldmann, V. W. M. Lai, W. R. Cullen, M. Ma, X. Lu and X. C. Le, Clin. Chem. 45, 1988 (1999).
  21. P. Apostoli, D. Bartoli, L. Alessio and J. P. Buchet, Occup. Environ. Med. 56, 825 (1999).
  22. K. A. Francesconi, R. Tanggaard, C. J. McKenzie and W. Goessler, Clin. Chem. 48, 92 (2002).
  23. M. Calatayud, V. Devesa and D. Vélez, Toxicology Letters 218, 70 (2013).
  24. M. Fragiadaki and R. M. Mason, Int. J. Exp. Pathol. 92, 143 (2011).
  25. S. Meran and R. Steadman, Int. J. Exp. Pathol. 92, 158 (2011).
  26. F. E. Pereira, J. D. Coffin and H. D. Beall, Toxicol. Appl. Pharmacol. 220, 164 (2007).
  27. V. Charoensuk, W. P. Gati, M. Weinfeld and X. C. Le, Toxicol. Appl. Pharmacol. 239, 64 (2009).
  28. S. L. Winski and D. E. Carter, J. Toxicol. Environ. Health 53, 345 (1998).
  29. E. Sánchez-López, R. R. Díez, J. R. Vita, S. R. Mateos, R. R. R. Díez, E. R. García, C. L. Barria, S. Mezzano, R. Selgas, J. Egido, A. Ortiz and M. Ruiz-Ortega, Nefrologia 29, 382 (2009).
  30. J. Zhang, X. Pan, N. Li, X. Li, Y. Wang, X. Liu, X. Yin and Z. Yu, Exp. Ther. Med. 7, 260 (2013).
  31. O. L. Valenzuela, D. R. Germolec, V. H. Borja-Aburto, J. Contreras-Ruiz, G. G. García-Vargas and L. M. Del Razo, Toxicol. Appl. Pharmacol. 222, 264 (2007).

Copyright @2019 | Designed by: Open Journal Systems Chile Logo Open Journal Systems Chile Support OJS, training, DOI, Indexing, Hosting OJS

Code under GNU license: OJS PKP