Vol 61 No 4 (2016): Journal of the Chilean Chemical Society
Original Research Papers
IMPLEMENTATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF INORGANIC ARSENIC SPECIES IN OCCUPATIONALLY EXPOSED HUMAN URINE SAMPLES AND ITS TOXIC EFFECTS ON EPITHELIAL CELLS OF RENAL COLLECTING TUBULE
Published
December 10, 2016
Keywords
- Arsenic,
- Speciation,
- Renal cells,
- Urine,
- Toxic effects
How to Cite
Roldán, N., Salinas Parra, N., Gonzalez, A. A., Cifuentes Araneda, F., Arias, H., Bravo, M., & Quiroz, W. (2016). IMPLEMENTATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF INORGANIC ARSENIC SPECIES IN OCCUPATIONALLY EXPOSED HUMAN URINE SAMPLES AND ITS TOXIC EFFECTS ON EPITHELIAL CELLS OF RENAL COLLECTING TUBULE. Journal of the Chilean Chemical Society, 61(4). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/114
Copyright (c) 2017 Nicole Roldán, Nicolás Salinas Parra, Alexis A. Gonzalez, Flavia Cifuentes Araneda, Helen Arias, Manuel Bravo, Waldo Quiroz
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Abstract
In this study a method was implemented for the determination of total As and its inorganic species in human urine by HPLC-HG-AFS. The method was applied to workers occupationally exposed to mining and thermoelectric operations. Results shows high levels of As in urine up to 188±4 μg L-1 and As(III) up to 10.7±0.6 μg L-1. Toxic effects of As(III) at the concentration levels founded in human urine were evaluated over the morphology and fibrosis of renal epithelial collecting duct cells in vitro. Our results suggest that urinary tract epithelial renal tubular cells exposed to this concentration of As(III) result in epithelial-mesenchymal transition.
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