JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 64 No 1 (2019): Journal of the Chilean Chemical Society
Original Research Papers

SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM

Sigrid Mennickent
Department of Pharmacy, Faculty of Pharmacy, University of Concepción
Marta De Diego
Department of Pharmacy, Faculty of Pharmacy, University of Concepción
Bernd Liser
Department of Pharmacy, Faculty of Pharmacy, University of Concepción
Lina Trujillo
Department of Pharmacy, Faculty of Pharmacy, University of Concepción
Published March 27, 2019
Keywords
  • Risperidone,
  • human serum,
  • liquid chromatography,
  • quantitative determination
How to Cite
Mennickent, S., De Diego, M., Liser, B., & Trujillo, L. (2019). SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM. Journal of the Chilean Chemical Society, 64(1). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/1032

Abstract

Risperidone, an antipsychotic drug, was determined in human serum, using an optimized liquid-liquid extraction for isolation from biological fluid, with ethyl acetate as extraction solvent. This sample extraction method was chosen to increase extraction efficiency and reduce matrix interferences. Quantitation was achieved by LC/DAD at 294 nm over the concentration range of 1 ng/mL to 60 ng/mL (r=0.999). Desipramine was used as internal standard (tR for Risperidone: 4.23; tR Desipramine: 7.31). Mobile phase was water: acetonitrile: triethilamine: glacial acetic acid (70:29.16:0.52: 0.33 v/v), using a Purosphere STAR RP-18e 250 x 4.5 mm (5μ) column. RSD, for the intra-assay study, was between 0.52 % - 2.09 % (n=3), and between 0.79 % -3.25 % (n=9) for the inter-assay. LOD was 0.40 ng/mL and LOQ was 0.87 ng/mL. Recovery percentage for the accuracy study was between 90.82% and 98.56% ((RSD ≤ 3.93). 

The method is simple, fast, precise, accurate, sensible and selective. This method was successfully applied to quantify risperidone in patient serum samples. In conclusion, the method is useful for quantitative determination of risperidone in human serum.

References

  1. G. Mc Evoy. AHFS Drug Information. American Society of Health- System Pharmacists, 2015.
  2. S. Sweetman. Martindale, Guía Completa de Consulta Farmacoterapéutica. Pharma Editores S.L., Barcelona, 2008.
  3. A.E. Balant-Gorgia, M. Gex-Fabry, C.H. Genet, L.P. Balant LP, Ther. Drug Monit. 21, 105-115., (1999).
  4. S.Y, Dong, J.S. Sook, Y. Young-Ran, J. Psycopharmacol., 147 300-305, (2013).
  5. P.M.Haddad, C. Brain, J. Scott, Patient Related Outcome Measures, 5, 43–62, (2014).
  6. S.C. Marcus, J. Zummo, A.R. Pettit, J. Stoddard, J.A. Doshi, JMCP, 21, 754-68, (2015).
  7. W. Yin Lam, P. Fresco, Bio Med Research International, 1, 1-12, (2015).
  8. D.I. Velligan, M. Sajatovic, A.Hatc, P. Kramata, J.P. Docherty, Patient Prefer. Adherence, 11,449-468, (2017).
  9. S.V. Phan, International Journal of Psychiatry in Medicine, 51,211-9, (2016).
  10. N.H. Sharaya, M.F Dorrell, N.A. Sciacca, JPP, 30, 37-41, (2017).
  11. M. Grundmann, I. Kacirova, R. Urinovska, Acta Pharm., 64, 387–401, (2014).
  12. S.M. Foroulan, A. Zarghi, A. Shafaati A. Iranian Journal of Pharmaceutical Research, 5, 37-70, (2006).
  13. B. Cabovska, A. Vinks, J, Chromatogr., B, 852, 497-504, (2007).
  14. H. Karadeniz, JJC, 13, 57-67, (2018).
  15. P. Torres, M.J. Sepúlveda, C.von Plessing, J. Chil .Chem., Soc., 56, 606- 609, (2011).
  16. K. Tonooka , L. Yoshida, K. Tomobe, Y. Kunisue, M. Terada, T.Shinozuka AJAC, 9, 88-97, (2018).
  17. I. Lakshmi Prasanna, G.T. Naidu, N. Fathima, I.E. Chakravarthy, G. Abdul Huq, IJPPR, 12, 148-159, (2018).

Copyright @2019 | Designed by: Open Journal Systems Chile Logo Open Journal Systems Chile Support OJS, training, DOI, Indexing, Hosting OJS

Code under GNU license: OJS PKP