JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 4 (2016): Journal of the Chilean Chemical Society
Original Research Papers

SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY

PDF
  • A. Müller-Sepúlveda,
  • M. E. Letelier,
  • B. San Martin,
  • I. Saavedra-Saavedra
A. Müller-Sepúlveda
Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile Center of Pharmacological and Toxicological Research, Program of Molecular and Clinical Pharmacology, Faculty of Medicine, University of Chile
M. E. Letelier
Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile
B. San Martin
Laboratory of Veterinary Pharmacology, Faculty of Veterinary Sciences, University of Chile
I. Saavedra-Saavedra
Center of Pharmacological and Toxicological Research, Program of Molecular and Clinical Pharmacology, Faculty of Medicine, University of Chile
Published May 29, 2017
Keywords
  • Flavonoids,
  • Catechin,
  • Rutin,
  • Apigenin-7-glucoside,
  • Luteolin-7-glucoside,
  • Baicalein,
  • Quercetin,
  • HPLC
    • ...More
    Less
How to Cite
Müller-Sepúlveda, A., Letelier, M. E., San Martin, B., & Saavedra-Saavedra, I. (2017). SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY. Journal of the Chilean Chemical Society, 61(4). Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/103

Copyright (c) 2017 A. Müller-Sepúlveda, M. E. Letelier, B. San Martin, I. Saavedra-Saavedra

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Abstract

A simple HPLC method for simultaneous determination of five flavonoids in human plasma was developed. Following addition of catechin as internal standard (IS), luteolin-7-glucoside, rutin, apigenin-7-glucoside, quercetin and baicalein were isolated from human plasma by liquid–liquid extraction with acetone. The chromatographic separation was achieved in a DAD-HPLC equipment on a reversed-phase C18 column using a gradient mobile phase consisting of 0.1% acetic acid + methanol: acetonitrile: acetic acid (90:10:1, v/v) and running at a flow rate of 1 mL/min. The effluent was monitored at a wavelength of 280 ηm, as well as IS was well separated from each other and free from interference from blank plasma and other components in plasma. The calibration curve was constructed by plotting peak area ratio of analytes to IS vs. concentration. The method showed good linearity over range of 5 - 30 μg/mL for the five standards used (r>0.996). The developed method may be used not only to identify the analytes assayed but, simultaneously measure plasma concentrations of these herbal compounds. 

PDF

References

  1. Quideau S, Deffieux D, Douat-Casassus C, Pouységu L. Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis. Angewandte Chemie International Edition. 586–621, (2011).
  2. Rah DK, Han D-W, Baek HS, Hyon S-H, Park J-C. Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol. Toxicol. Lett.155: 269–75, (2005).
  3. Sies H. Polyphenols and health : Update and perspectives. Arch Biochem Biophys. 501:2-5, (2010).
  4. Tapiero H, Tew KD, Ba GN, Mathé G. Polyphenols: do they play a role in the prevention of human pathologies? Biomed Pharmacother. 56(4): 200-207, (2002).
  5. Martins S, Mussatto SI, Martínez-Avila G, Montañez-Saenz J, Aguilar CN, Teixeira JA. Bioactive phenolic compounds: Production and extraction by solid-state fermentation. A review. Biotechnol Adv. 29(3): 365-373, (2011).
  6. Scalbert A, Williamson G. Dietary Intake and Bioavailability of Polyphenols. J Nutr. 130: 2073-2085, (2000).
  7. Quideau S, Deffieux D, Douat-Casassus C, Pouységu L. Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis. In: Angewandte Chemie International Edition.Vol 50: 586-621, (2011).
  8. Rahman I, Biswas SK, Kirkham PA. Regulation of inflammation and redox signaling by dietary polyphenols. Biochem Pharmacol. 72(11): 1439-1452, (2006;).
  9. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How are they linked? Free Radic Biol Med. 49(11): 1603-1616, (2010).
  10. Del Rio D, Costa LG, Lean MEJ, Crozier A. Polyphenols and health: what compounds are involved? Nutr Metab Cardiovasc Dis. 20(1): 1-6, (2010).
  11. Pignatelli P, Ghiselli A, Buchetti B, et al. Polyphenols synergistically inhibit oxidative stress in subjects given red and white wine. Atherosclerosis. 188(1): 77-83, (2006).
  12. Wagner H. Synergy research : Approaching a new generation of phytopharmaceuticals. Fitoterapia. 82:34-37, (2011).
  13. Ulrich-Merzenich G, Panek D, Zeitler H, Vetter H, Wagner H. Drug development from natural products: exploiting synergistic effects. Indian J Exp Biol. 48(3): 208-219, (2010).
  14. Tallarida RJ. Drug synergism: its detection and applications. J Pharmacol Exp Ther. 298(3): 865-872, (2001).
  15. Williamson G, Manach C. Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention studies. Am J Clin Nutr. 81(1 Suppl): 243S - 255S, (2005).
  16. Bell JR, Donovan JL, Wong R, et al. (+)-Catechin in human plasma after ingestion of a single serving of reconstituted red wine. Am J Clin Nutr. 71(1): 103-108, (2000).
  17. Maiani G, Serafini M, Salucci M, Azzini E, Ferro-Luzzi A. Application of a new high-performance liquid chromatographic method for measuring select polyphenols in human plasma. J Chromatogr B. 692(2): 311-317, (1997).
  18. Abrahamse SL, Kloots WJ, van Amelsvoort JMM. Absorption, distribution, and secretion of epicatechin and quercetin in the rat. Nutr Res. 25(3): 305-317, (2005).
  19. Fu T, Liang J, Han G, Lv L, Li N. Simultaneous determination of the major active components of tea polyphenols in rat plasma by a simple and specific HPLC assay. J Chromatogr B Analyt Technol Biomed Life Sci. 875(2): 363-367, (2008).
  20. Yang Z, Zhu W, Gao S, et al. Simultaneous determination of genistein and its four phase II metabolites in blood by a sensitive and robust UPLC-MS/ MS method: Application to an oral bioavailability study of genistein in mice. J Pharm Biomed Anal. 53(1): 81-89, (2010).
  21. Yang GJ, Liu P, Qu XL, et al. The simultaneous separation and determination of six flavonoids and troxerutin in rat urine and chicken plasma by reversed-phase high-performance liquid chromatography with ultraviolet-visible detection. J Chromatogr B Anal Technol Biomed Life Sci. 856(1-2): 222-228, (2007).
  22. Hamed AI, Said R Ben, Kontek B, et al. LC–ESI-MS/MS profile of phenolic and glucosinolate compounds in samh flour (Mesembryanthemum forsskalei Hochst. ex Boiss) and the inhibition of oxidative stress by these compounds in human plasma. Food Res Int. 85:282-290, (2016).
  23. European Medicines Agency. Guideline on Bioanalytical Method Validation. (2012).
  24. Marston A. Role of advances in chromatographic techniques in phytochemistry. Phytochemistry. 68(22-24): 2786-2798, (2007).
  25. Simirgiotis MJ, Schmeda-Hirschmann G. Determination of phenolic composition and antioxidant activity in fruits, rhizomes and leaves of the white strawberry (Fragaria chiloensis spp. chiloensis form chiloensis) using HPLC-DAD–ESI-MS and free radical quenching techniques. J Food Compos Anal. 23(6): 545-553, (2010).
  26. Vasco C, Riihinen K, Ruales J, Kamal-Eldin A. Phenolic compounds in Rosaceae fruits from Ecuador. J Agric Food Chem. 57(4): 1204-1212, (2009).
  27. Abad-García B, Berrueta L a, Garmón-Lobato S, Gallo B, Vicente F. A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. J Chromatogr A. 1216(28): 5398-5415, (2009).
  29. Sakakibara H, Honda Y, Nakagawa S, Ashida H, Kanazawa K. Simultaneous determination of all polyphenols in vegetables, fruits, and teas. J Agric Food Chem. 51(3): 571-581, (2003).
  30. Zhang W, Han F, He J, Duan C. HPLC-DAD-ESI-MS/MS Analysis and Antioxidant Activities of Nonanthocyanin Phenolics in Mulberry (Morus alba L.). J Food Sci. 73(6): C512-C518, (2008).
  31. Funes L, Fernández-Arroyo S, Laporta O, et al. Correlation between plasma antioxidant capacity and verbascoside levels in rats after oral administration of lemon verbena extract. Food Chem. 117(4): 589-598, (2009).
  32. D’Archivio M, Filesi C, Varì R, Scazzocchio B, Masella R. Bioavailability of the polyphenols: status and controversies. Int J Mol Sci. 11(4): 1321-1342, (2010).

Copyright @2019 | Designed by: Open Journal Systems Chile Logo Open Journal Systems Chile Support OJS, training, DOI, Indexing, Hosting OJS

Code under GNU license: OJS PKP