JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 71 No 1 (2026): Journal of The Chilean Chemical Society - Special Issue in Tribute to Professor Mario Silva (In Memoriam)
Original Research Papers

DETERMINATION IN VITRO OF THE ANTIBACTERIAL ACTIVITY OF CHILEAN NATIVE ESSENTIAL OILS AGAINST PSEUDOMONAS SYRINGAE PV. ACTINIDIAE AND PSEUDOMONAS SYRINGAE PV. SYRINGAE ISOLATES

Felipe Moraga-Nicolás
UNIVERSIDAD DE LA FRONTERA
Published August 2, 2026
Keywords
  • Pseudomonas syringae pv. actinidiae, Essential oils, Antibacterial activity
How to Cite
Moraga-Nicolás, F., Hormazábal, E., Mutis, A., Quiroz, A., Celi, J., Guerrero Contreras, J., & Pérez Fuentealba, S. (2026). DETERMINATION IN VITRO OF THE ANTIBACTERIAL ACTIVITY OF CHILEAN NATIVE ESSENTIAL OILS AGAINST PSEUDOMONAS SYRINGAE PV. ACTINIDIAE AND PSEUDOMONAS SYRINGAE PV. SYRINGAE ISOLATES. Journal of the Chilean Chemical Society, 71(1), 6490 - 6494. Retrieved from https://jcchems.com/index.php/JCCHEMS/article/view/3072

Abstract

Pathogenic bacteria Pseudomonas syringae pv. actinidiae (Psa) and Pseudomonas syringae pv. syringae (Pss) causes bacterial canker disease in kiwifruit orchards. In this regard antibacterial activities of Chilean native species Peumus boldus (Molina), Laurelia sempervirens (Ruiz & Pav. Tul.), Cryptocarya alba (Mol) Looser essential oils, α-pinene, eucalyptol and p-cymene showed efficacy against Psa and Pss strains by disc diffusion method and liquid culture assay. The P. boldus essential oil at 7.0 mg/disc was significantly more effective than L. sempervirens and C. alba essential oil at the same concentration, using streptomycin as positive control. Additionally, α-pinene and p-cymene showed a remarkable effect in the inhibition of all strains tested at the same concentration. On the other hand, p-cymene showed a notable antibacterial activity similar to streptomycin against Psa. The P. boldus essential oils minimal inhibitory concentrations (MIC) and minimal bactericidal (MBC) concentration ranged from 312.5  to 625 ppm against Psa. The efficacy of P. boldus EO was comparable to streptomycin in in vitro conditions, offering potential as a sustainable alternative to integrated control of Psa in the kiwifruit industry.

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References

  1. Scortichini et al., “Pseudomonas syringae pv. actinidiae: a re-emerging, multi-faceted, pandemic pathogen”, Mol. Plant Pathol. 13 631–640, 2012.
  2. Ferrante and Scortichini, “Molecular and phenotypic features of Pseudomonas syringae pv. actinidiae isolated during recent epidemics of bacterial canker on yellow kiwifruit (Actinidia chinensis) in central Italy”. Plant Pathol. 59:954–62, 2010.
  3. Ferrante and Scortichini, “Redefining the global populations of Pseudomonas syringae pv. actinidiae based on pathogenic, molecular and phenotypic characteristics”. Plant Pathol ;64: 51–62, 2015.
  4. McCann et al., “Origin and Evolution of the Kiwifruit Canker Pandemic”, Genome Biology and Evolution, 2017. doi.org/10.1093/gbe/evx055.
  5. Reglinski et al., “Using fundamental knowledge of induced resistance to develop control strategies for bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae”. Frontiers in Plant Science. 2013. doi: 10.3389/fpls.2013.00024
  6. Wicaksono et al., “Biological control of Pseudomonas syringae pv. actinidiae (Psa), the causal agent of bacterial canker of kiwifruit, using endophytic bacteria recovered from a medicinal plant”. Biological Control. 2018. doi.org/10.1016/j.biocontrol.2017.03.003
  7. Vavala et al., “Antibacterial activity of essential oils mixture against PSA”. Natural Product Research. 2015. doi.org/10.1080/14786419.2015.1022543.
  8. Moraga-Nicolás et al., “Rhodolirium andicola: a new renewable source of alkaloids with acetylcholinesterase inhibitory activity, a study from nature to molecular docking”. Revista Brasileira de Farmacognosia 28. 2018. DOI: 10.1016/j.bjp.2017.11.009
  9. Touma et al, “The Chemical Compositions of Essential Oils Derived from Cryptocarya alba and Laurelia sempervirens Possess Antioxidant, Antibacterial and Antitumoral Activity Potential.” Molecules 2020. doi:10.3390/molecules25235600
  10. Moraga-Nicolás et al., “Berberis microphylla G. Forst: a Chilean renewable source of cholinesterases and prolyl oligopeptidase inhibitors”. Nat Prod Res. 2025. DOI: 10.1080/14786419.2025.2588804.
  11. Giovanardi et al., “Sustainable and innovative biological control strategies against Pseudomonas syringae pv. tomato, Pseudomonas savastanoi pv. phaseolicola and Xanthomonas spp. affecting vegetable crops: a review”. Front. Plant Sci. 2025. doi: 10.3389/fpls.2025.1536152
  12. Toledo et al., “Chemical composition and antibacterial activity of Laureliopsis philippiana (Looser) essential oil” Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2014.
  13. Sonboli et al.,” Antibacterial and antioxidant activity and essential oil composition of Grammosciadium scabridum Bioss. from Iran”, Z Naturforsch, 60: 534 – 538, 2005.
  14. Longaray et al., “Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil”, Food Chem 100: 603 – 608, 2007.
  15. Song et al., “Antibacterial Activity of Cinnamaldehyde and Estragole Extracted from Plant Essential Oils against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit”. Plant Pathol J. 2016. doi: 10.5423/PPJ.NT.01.2016.0006.
  16. Iacobellis et al.,” Antibacterial Activity of Cuminum cyminum L. and Carum carvi L. Essential Oils”, J. Agric. Food Chem. 2005, doi.org/10.1021/jf0487351.
  17. de Souza et al., “Evaluation of the volatile composition, toxicological and antioxidant potentials of the essential oils and teas of commercial Chilean boldo samples”, Food Res Int. 2019. doi: 10.1016/j.foodres.2018.12.059.
  18. King, E.O., Ward, M.K., & Raney, D.E. Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine, 44(2), 301-307, 1954.
  19. Biondini et al., “Pseudomonas syringae pv. actinidiae detection in kiwifruit plant tissue and bleeding sa”, Annals of Applied Biology, 2013. DOI: 10.1111/aab.12001.
  20. Sorensen et al, “PCR detection of cyclic lipodepsinonapeptide-producing Pseudomonas syringae pv. syringae and similarity of strains”, Applied and Environmental Microbiology, 1998. DOI: 10.1128/aem.64.1.226-230.1998
  21. Babushok Viet al, “Development of a database of gas chromatographic retention properties of organic compounds”. J Chromatogr A 1157: 414 – 421, 2007.
  22. National Committee for Clinical Laboratory Standard. In Performance standards for antimicrobial susceptibility testing: 11th informational supplement. NCCLS document M100-S11, Wayne, PA, USA. 2001.
  23. Jerckovic et al., “The impact of both the season of collection and drying on the volatile constituents of Origanum vulgare. L. spp. Hirtum grown wild in Croatia”. Int. J. Food Sci. Technol 36: 649 – 654, 2001.
  24. Cimanga K et al., “Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo”. J Ethnopharmacol 79: 213 – 220, 2002
  25. Almas et al., “Effect of Geographical location on yield and chemical composition of essential oils from three Eucalyptus species growing in Tanzania”. Asian Journal of Traditional Medicines. 14. 1-12, 2019
  26. Donahue J et al, “Geographic variation in stem-xylem terpene chemistry in native populations of Pinus Gregii Engelm”. Forest Genetics 2: 217 – 225, 1995
  27. Júnior et al., “Antimicrobial activity of essential oil of Piper aduncum L. (Piperaceae)”, J Med Plants Res 6: 3800 – 3805, 2012.
  28. Vieitez et al., “Antioxidant and antibacterial activity of different extracts from herbs obtained by maceration or supercritical technology”, The Journal of Supercritical Fluids, 2017. DOI: 10.1016/j.supflu.2017.09.025.
  29. Montenegro et al., “Actividad antimicrobiana del extracto acetato de etilo y aceite esencial de corteza de Laurelia sempervirens contra bacterias multirresistentes”, Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 11. 306-315, 2012.
  30. Bagamboula, et al., “Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri” Food Microbiology, 2004. https://doi.org/10.1016/S0740-0020(03)00046-7.
  31. Kiskó and Roller, “Carvacrol and p-cymene inactivate Escherichia coli O157:H7 in apple juice”, BMC Microbiol, 2005. DOI: 10.1186/1471-2180-5-36.
  32. Nikaido, “Molecular basis of bacterial outer membrane permeability revisited” Microbiol Mol Biol Rev. 2003. DOI: 10.1128/MMBR.67.4.593-656.2003
  33. Cristani M. et al., “Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity” Journal of Agricultural and Food Chemistry, 2007. DOI: 10.1021/jf070094x.

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