ANTIMICROBIAL ACTIVITIES OF MYCORRHIZIN A PRODUCED BY LIQUID FERMENTATIONS OF AN ENDOPHYTIC PEZICULA CINNAMOMEA (NA-78) ISOLATED FROM NOTHOFAGUS ALPINA IN CHILE
- antimicrobial activity,
- chilean endophytc fungi,
- mycorrhizin A,
- Nothofagus alpina,
- Pezicula cinnamomea
- submerged fermentation ...More
Copyright (c) 2026 SChQ
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Abstract
The present work is part of a program aimed at searching for bioactive from Chilean fungi. In this opportunity, we report the study on Chilean endophytic fungi, identified as Pezicula cinnamomea (strain Na-78) isolated from Nothofagus alpina, collected in the Ñuble Region-Chile. Mycelial submerged fermentations of P. cinnamomea-78 were performed. Two types of extracts were obtained, EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extract was more active than MeOH-extract. A large-scale fermentation of P. cinnamomea-78 and chromatographic and spectroscopic methods allowed to isolate and identify, from EtOAc-extract, a bioactive compound named Mycorrhizin A. This compound showed antibacterial activity against B. cereus, S. aureus and S. aureus MRSA. Also, Mycorrhizin A was active toward filamentous fungi, such as A. niger, B. cinerea and F. oxysporum. Assays showed that Mycorrhizin A exhibited a bactericidal effect against B. subtilis and S. aureus at 10 µg/mL and bacteriostatic activity at 25 µg/mL. Also, Mycorrhizin A showed a fungistatic/fungicidal effect toward A. niger and B. cinerea at 25-100 µg/mL, respectively. The study established that Mycorrhizin A was bacteriostatic against S. aureus MRSA and fungistatic against F. oxysporum at 200 µg/mL. This study constitute the first report of P. cinnamomea for the genus Nothofagus. Our research has revealed a new antibacterial activity of mycorrhizal A and has broadened the spectrum of antifungal activity.
References
- T. Anke, H. Anke, G. Erkel, Screening 2, 4, (2001)
- T. Anke, J. Antibiot, 73, 655, (2020)
- E. Martinez-Klimova, K. Rodríguez-Peña, S. Sánchez, Biochem. Pharmacol., 134, 1, (2017)
- B. Guo, Y. Wang, X. Sun, K.Tang, Appl. Biochem. Microbiol. 44, 136, (2008)
- M. Rajamanikyam, V. Vadlapudi, R. Amanchy, S. M. Upadhyayula, Braz. Arch. Biol. Technol. 60,1, (2017)
- P. Tiwari, S. Thakkar, L. Dufossé, Crit. Rev. Biotechnol. 45, 164, (2025)
- B. Schulz, C. Boyle, Mycol. Res. 109, 661, (2005)
- S. Kusari, C. Hertweck,; M. Spiteller, Chem. Biol.,19, 792, (2012)
- M. Rashmi, J.S. Kushveer, V.V. Sarma, Mycosphere 10, 798, (2019)
- C. S. Bhunjun, C. Phukhamsakda, K. D. Hyde, E. H. C. McKenzie, R. K. Saxena, Q. Li, Fungal Divers. 125, 73, (2024)
- R. Devi, R. Verma, R. Dhalaria, A. Kumar, D. Kumar, S. Puri, M. Thakur, S. Chauhan, P. P. Chauhan, E. Nepovimova, et al. Planta, 257, 70, (2023)
- A. R. Khattab, M. A. Farag, Crit. Rev. Biotechnol. 42, 403, (2022)
- C. Mili, Arch. Microbiol. 205, 254, (2023)
- J. Jin, Q. Zhao,; X.M. Zhang, W.J. Li, J. Microbiol. 38, 103, (2018)
- S.K. Deshmukh, M.K. Gupta, Prakash, V. S. Saxena, J. Fungi. 4, 77, (2018)
- R.M.T. Kouipou, F.F. Boyom, J. Fungi, 5, 43, (2019)
- D. Popli, V. Anila, A.B. Subramanyama, M.N. Namratha, V.R. Ranjitha, S.N. Raoa, V. Ravishankar, M. Govindappaa, Artif. Cell Nanomed. Biotechnol. 46, 676, (2018)
- R.H. Zheng, S.J. Li, X. Zhang, C.Q. Zhao, Int. J. Mol. 22, 959, (2021)
- G. Dhayanithy, K. Subban, J. Chelliah, BMC Microbiol. 19, 22, (2019)
- P. Aqueveque, T. Anke, O. Sterner, Zeitschrift für Naturforschung C, 57, 257, (2002)
- P. Aqueveque, C. Céspedes, J. Alarcón, G. Schmeda-Hirschmann, J. Cañumir, J. Becerra, M. Silva, O. Sterner, R. Radrigán, M. Aranda, Crop Protection 89, 95, (2016)
- P. Aqueveque, C. Céspedes, J. Becerra, M. Dávila, O. Sterner, Zeitschrift für Naturforschung C, 70, 97, (2015)
- P. Aqueveque, T. Anke, K. Sáez, M. Silva, J. Becerra, Planta Med. 76, 1787, (2010)
- H. Valenzuela, D. Aqueveque-Jara, M. Sanz, M. Ocampo, K. Henríquez-Aedo, M. Aranda, P. Aqueveque, J. Fungi. 12, 77, (2026)
- Q. Chen, J. Wang, Y. Gao, X. Gao, P. Yan,. Microorganisms 11, 2331, (2023)
- T. Xie, S. Shen, R. Hu, W. Li, J. Wang, Phytopathology, 113, 1839, (2023)
- V. Rojas, V. Mierau, T. Anke, O. Sterner, J. Antibiot. 59, 57, (2006)
- M. Kettering, C. Valdivia, O. Sterner, H. Anke, E. Thines, J. Antibiot. 58, 390, (2005)
- J. Liermann, H. Kolshorn, H.Anke, E. Thines, T. Opatz, J. Nat. Prod, 71, 1654, (2008)
- S. Lu, W. Sun, J. Meng, A. Wang, X. Wang, J. Tian, X. Fu, J. Dai, Y. Liu, D. Lai, J. Agric. Food Chem. 63, 3501, (2015)
- J. Wang, G. Wang,Y. Zhang, B. Zheng, C. Zhang, L.Wang, World J. Microbiol. Biotechnol, 30, 2639, (2014)
- D.-H. Yan, X. Song, H. Li, T. Luo, G. Dou, G. Strobel, J. Fungi (Basel), 4, 65, (2018)
- Ö. Demir, H.Zeng,; B. Schulz, H. Schrey, M. Steinert, M.Stadler, F. Surup, Biomolecules, 13, 1632, (2023)
- Y.-C. Shu, Z.-Y Zhang, X.-M. Fu, W.-L. Zeng, G.-L. Zhu, X.-Q. Yang, S.-D. Xie, W.-H. Wang, P. Zhao, J. Asian Nat. Prod. Res. 27, 267, (2025)
- H. Hussain, C. Kliche-Spory, A. Al-Harrasi, A. Al-Rawahi, G. Abbas, IR. Green, B. Schulz, K. Krohn, A. Shah, Asian Pac J Trop Med. 7, S224, (2014)
- V.M. Dembitsky, G.A. Tolstikov, Chem Sustain Dev, 11, 567, (2003)
