PRODUCTION OF METALLIC NANOPARTICLES FROM FUNGAL METABOLITES OF Gymnopilus junonius WITH ANTIMICROBIAL POTENTIAL
- Nanotecnología,
- Química verde
Copyright (c) 2026 SChQ
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Abstract
The aim of this study was to evaluate the antimicrobial properties of silver nanoparticles (AgNPs) synthesized using the metabolites of the fungus Gymnopilus junonius, grown on stumps of Pino spp. and Eucalipto spp. as substrates. Samples were collected from three locations in the Biobío Region to extract their metabolites and optimize the biosynthesis of nanoparticles, which were characterized using UV-VIS spectroscopy, TEM, and FTIR. AgNPs derived from pine showed a smaller average size (10–15 nm) and greater uniformity, whereas those from eucalyptus displayed a broader size range (20–30 nm), highlighting the impact of different metabolites on the synthesis process. Antimicrobial tests revealed that both AgNPs were effective against gram-positive (Staphylococcus aureus) and gram-negative bacteria, as well as pathogenic fungi (Candida albicans and C. glabrata). However, AgNPs derived from pine demonstrated greater efficacy, likely due to their smaller size, which facilitates interaction with microbial membranes. This study highlights the capability of the fungus Gymnopilus junonius as a sustainable biofactory for producing nanoparticles with antimicrobial properties, emphasizing its potential application in microbial infection treatments and agriculture. Furthermore, this approach provides an eco-friendly alternative to the use of conventional chemical agents in nanoparticle synthesis.
References
[2] S. Gurunathan, J. H. Park, J. W. Han, J. H. Kim, Int. J. Nanomed. 10, 4203, (2015)
[3] L. N. Lewis, Chem. Rev. 93, 2693, (1993)
[4] A. G. Rodrigues, L. Y. Ping, P. D. Marcato et al., Appl. Microbiol. Biotechnol. 97, 775, (2013)
[5] P. Sanguiñedo, M. B. Estevez, R. Faccio, S. Alborés, Mundo Nano 12, 101, (2018)
[6] G. L. Gómez, Investig. Discapacidad 2, 18, (2013)
[7] D. Spadaro et al., Appl. Surf. Sci. 256, 3812, (2010)
[8] S. K. Dorcheh, K. Vahabi en Springer eBooks, Springer, 2016; pp. 1-20.
[9] M. Rai, S. Bonde, P. Golinska, J. Trzcinska-Wencel, A. Gade, K. A. Abd-Elsalam, S. Shende, S. Gaikwad, A. P. Ingle, J. Fungi 7, 139, (2021)
[10] E. Castro-Longoria, D. Garibo-Ruiz, S. Martínez-Castro en Fungal Nanotechnology, Springer, Cham, 2017; pp. 235-261.
[11] K. Quester, M. Avalos-Borja, E. CastroLongoria, Micron 54, 1, (2013)
[12] E. Castro-Longoria, A. R. Vilchis-Nestor, M. Avalos-Borja, Colloids Surf. B 83, 42, (2011)
[13] L. E. García-Marín, E. Castro-Longoria, Mundo Nano 16, 69780, (2023)
[14] M. A. Alghuthaymi, A. Kalia, K. Bhardwaj, P. Bhardwaj, K. A. Abd-Elsalam, M. Valis, K. Kuca, J. Fungi 7, 48, (2021)
[15] R. Prasad, Nanotecnología fúngica: aplicaciones en agricultura, industria y medicina, Springer, 2017.
[16] E. Castro Longoria, Farmacología 1, 6, (2022)
[17] H. Alarcón, M. Tolmos, N. Villacrés, E. Huarote, Rev. Soc. Quím. Perú 87, 237, (2021)
[18] S. N. Rai, D. Mishra, P. Singh, M. P. Singh, E. Vamanu, A. Petre, Curr. Pharm. Des. 29, 1002, (2023)
[19] M. Volland, Factores y mecanismos de toxicidad de las nanopartículas metálicas (Tesis Doctoral), Universidad de Cádiz, 2017.
[20] R. Vazquez-Muñoz, M. Avalos-Borja, E. Castro-Longoria, PLoS One 9, e108876, (2014)
[21] D. G. Brown, D. W. Denning, N. A. R. Gow, S. M. Levitz, M. G. Netea, T. C. Blanco, Sci. Transl. Med. 4, 165rv13, (2012)
[22] M. C. Fisher, N. J. Hawkins, D. Sanglard, S. J. Gurr, Science 360, 739, (2018)
[23] J. R. Perfect, Nat. Rev. Drug Discov. 16, 603, (2017)
[24] Y. Lee, E. Puumala, N. Robbins, L. E. Cowen, Chem. Rev. 121, 3390, (2020)
[25] A. Chakrabarti, S. Singh, Expert Rev. Anti-Infect. Ther. 18, 551, (2020)
[26] L. A. Camacho Silvas, Rev. Esp. Salud Publica 97, e202302013, (2023)
[27] M. Ángel, S. Valdés, Rev. Habanera Cienc. Méd. 6, 402, (2017)
[28] V. M. Chávez-Jacobo, TIP Rev. Esp. Cienc. Quím. Biol. 23, e20200202, (2020)
[29] C. Lozano, C. Torres, Enferm. Infecc. Microbiol. Clin. 35, 2, (2017)
[30] M. C. Fariñas, L. Martínez-Martínez, Enferm. Infecc. Microbiol. Clin. 31, 402, (2013)
[31] S. Morales, M. A. Gallego, J. M. Vanegas, J. N. Jiménez, CES Med. 32, 203, (2018)
[32] T. J. Gintjee, M. A. Donnelley, G. R. Thompson, J. Fungi 6, 28, (2020)
[33] E. Boros, P. J. Dyson, G. Gasser, Chem 6, 41, (2020)
[34] F. Li, J. G. Collins, F. R. Keene, Chem. Soc. Rev. 44, 2529, (2015)
[35] G. Hatfield, L. Brady, J. Pharm. Sci. 58, 1298, (1969)
[36] P. Aqueveque, T. Anke, K. Saéz, M. Silva, J. Becerra, Planta Med. 76, 1787, (2010)
[37] S. Bhatnagar, T. Kobori, D. Ganesh, K. Ogawa, H. Aoyagi, Nanomaterials 9, 7, (2019)
[38] R. M. Bernal, M. Guzman U., Rev. Biomed. 4, 1, (1984)
[39] S. Iravani, H. Korbekandi, S. V. Mirmohammadi, B. Zolfaghari, Res. Pharm. Sci. 9, 385, (2014)
[40] M. Wu, H. Guo, L. Liu, Y. Liu, L. Xie, Int. J. Nanomed. 14, 4247, (2019)
[41] T. C. Dakal, A. Kumar, R. S. Majumdar, V. Yadav, Front. Microbiol. 7, 1831, (2016)
[42] A. Salleh, R. Naomi, N. D. Utami, A. W. Mohammad, E. Mahmoudi, N. Mustafa et al., Nanomaterials 10, 1566, (2020)
[43] T. Bruna, F. Maldonado-Bravo, P. Jara, N. Caro, Int. J. Mol. Sci. 22, 7202, (2021)
[44] M. J. Hajipour, K. M. Fromm, A. A. Ashkarran et al., Trends Biotechnol. 30, 499, (2012)
